mouse alkaline phosphatase Search Results


rabbit anti mouse igg antibodies  (Jackson Immuno)
93
Jackson Immuno rabbit anti mouse igg antibodies
Rabbit Anti Mouse Igg Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alp antibody  (R&D Systems)
93
R&D Systems alp antibody
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Alp Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/pmc10830172-66-32-36?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
alp antibody - by Bioz Stars, 2026-07
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mouse igg isotype controls  (R&D Systems)
94
R&D Systems mouse igg isotype controls
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Mouse Igg Isotype Controls, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/pmc06538843-55-44-48?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
mouse igg isotype controls - by Bioz Stars, 2026-07
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alkaline phosphatase ap conjugated anti mouse igg antibody  (Jackson Immuno)
93
Jackson Immuno alkaline phosphatase ap conjugated anti mouse igg antibody
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Alkaline Phosphatase Ap Conjugated Anti Mouse Igg Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/us09289488-455-44-51?v=Jackson+Immuno
Average 93 stars, based on 1 article reviews
alkaline phosphatase ap conjugated anti mouse igg antibody - by Bioz Stars, 2026-07
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ap  (Jackson Immuno)
93
Jackson Immuno ap
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Ap, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/us11124799-871-0-3?v=Jackson+Immuno
Average 93 stars, based on 1 article reviews
ap - by Bioz Stars, 2026-07
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alkaline phosphatase conjugated goat anti mouse secondary antibody  (Novus Biologicals)
90
Novus Biologicals alkaline phosphatase conjugated goat anti mouse secondary antibody
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Alkaline Phosphatase Conjugated Goat Anti Mouse Secondary Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/pmc05075610-48-20-26?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews
alkaline phosphatase conjugated goat anti mouse secondary antibody - by Bioz Stars, 2026-07
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antialpl  (OriGene)
90
OriGene antialpl
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Antialpl, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/pm28436570-52-12-31?v=OriGene
Average 90 stars, based on 1 article reviews
antialpl - by Bioz Stars, 2026-07
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α mouse alkaline phosphatase conjugated antibody  (Rockland Immunochemicals)
93
Rockland Immunochemicals α mouse alkaline phosphatase conjugated antibody
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
α Mouse Alkaline Phosphatase Conjugated Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/pmc08989999-384-3-7?v=Rockland+Immunochemicals
Average 93 stars, based on 1 article reviews
α mouse alkaline phosphatase conjugated antibody - by Bioz Stars, 2026-07
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goat anti mouse antibodies  (Rockland Immunochemicals)
88
Rockland Immunochemicals goat anti mouse antibodies
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Goat Anti Mouse Antibodies, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/pmc03568590-158-26-29?v=Rockland+Immunochemicals
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goat anti mouse antibodies - by Bioz Stars, 2026-07
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jackson immunoresearch cat  (Jackson Immuno)
91
Jackson Immuno jackson immunoresearch cat
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Jackson Immunoresearch Cat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/pm34748281-428-25-25?v=Jackson+Immuno
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mouse igg alkaline phosphatase conjugate  (Jackson Immuno)
92
Jackson Immuno mouse igg alkaline phosphatase conjugate
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Mouse Igg Alkaline Phosphatase Conjugate, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+alkaline+phosphatase/us09587024-534-6-11?v=Jackson+Immuno
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mouse igg alkaline phosphatase conjugate - by Bioz Stars, 2026-07
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alkaline phosphatase affinipure goat anti mouse igg  (Jackson Immuno)
95
Jackson Immuno alkaline phosphatase affinipure goat anti mouse igg
Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase <t>(ALP)</t> and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), <t>and</t> <t>collagen</t> type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.
Alkaline Phosphatase Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alkaline phosphatase affinipure goat anti mouse igg - by Bioz Stars, 2026-07
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Image Search Results


Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase (ALP) and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), and collagen type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.

Journal: Bone & Joint Research

Article Title: Down-expression of miR-494-3p in senescent osteocyte-derived exosomes inhibits osteogenesis and accelerates age-related bone loss via PTEN/PI3K/AKT pathway

doi: 10.1302/2046-3758.132.BJR-2023-0146.R2

Figure Lengend Snippet: Effects of senescent MLO-Y4 cell-derived exosomes (abbreviated tert-Butyl hydroperoxide (TBHP)-exos in the figure) on osteogenic differentiation of MC3T3-E1 cells. a) Cell Counting Kit 8 (CCK-8) assay of MC3T3-E1 cells treated with different concentrations (20, 100, or 300 μg/ml) of senescent MLO-Y4 cell-derived exosomes for different lengths of time (0, 24, 48, or 72 hours). b) Alkaline phosphatase (ALP) and Alizarin Red S staining of MC3T3-E1 cells on days 7, 14, and 21 after treatment with the indicated concentrations of exosomes obtained from senescent MLO-Y4 cells. c) and d) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), and collagen type I α1 (Col-I) expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The primary antibodies used were P16 (1:1000, ab51243; Abcam, USA), Runt-related transcription factor 2 (Runx2, 1:1,000, 12,556 S; Cell Signaling Technology), collagen type I α1 (Col-I, 1:1,000, 72,026 T; Cell Signaling Technology), ALP antibody (1:1,000, AF2910; R&D Systems, USA), AKT (1:1,000, 60203-2-Ig; Proteintech, USA), phosphorylated (p)-AKT (1:1,000, 66444-1-Ig; Proteintech), PI3K (1:1,000, 20584-1-AP; Proteintech), PTEN (1:1,000, 22034-1-AP; Proteintech), CD9 (1:1,000, 60232-1-Ig; Proteintech), and CD63 (1:1,000, 67605-1-Ig; Proteintech), while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:5,000, 60004-1-Ig; Proteintech) was used as a loading control.

Techniques: Derivative Assay, Cell Counting, CCK-8 Assay, Staining, Western Blot, Expressing, Comparison

miR-494-3p in MLO-Y4 cell-derived exosomes regulated osteogenic differentiation of MC3T3-E1 cells via the phosphatase and tensin homolog (PTEN)/phosphoinositide 3-kinase (PI3K)/AKT pathway. a) Alkaline phosphatase (ALP) and Alizarin Red S staining of MC3T3-E1 cells on day 21 in different treatment groups. The magnification is 10× (lower part). b) and c) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), collagen type I α1 (Col-I), p-AKT, AKT, PI3K, and PTEN expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase; miR, microRNA; TBHP, tert-Butyl hydroperoxide.

Journal: Bone & Joint Research

Article Title: Down-expression of miR-494-3p in senescent osteocyte-derived exosomes inhibits osteogenesis and accelerates age-related bone loss via PTEN/PI3K/AKT pathway

doi: 10.1302/2046-3758.132.BJR-2023-0146.R2

Figure Lengend Snippet: miR-494-3p in MLO-Y4 cell-derived exosomes regulated osteogenic differentiation of MC3T3-E1 cells via the phosphatase and tensin homolog (PTEN)/phosphoinositide 3-kinase (PI3K)/AKT pathway. a) Alkaline phosphatase (ALP) and Alizarin Red S staining of MC3T3-E1 cells on day 21 in different treatment groups. The magnification is 10× (lower part). b) and c) Western blotting and grayscale analysis of ALP, Runt-related transcription factor 2 (Runx2), collagen type I α1 (Col-I), p-AKT, AKT, PI3K, and PTEN expression in MC3T3-E1 cells in different treatment groups. Data are shown as column charts and the p-values were calculated by one-way analysis of variance with Sidak’s multiple comparison tests. The p-values were specified only when p < 0.05 (statistically significant). GD, glyceraldehyde 3-phosphate dehydrogenase; miR, microRNA; TBHP, tert-Butyl hydroperoxide.

Article Snippet: The primary antibodies used were P16 (1:1000, ab51243; Abcam, USA), Runt-related transcription factor 2 (Runx2, 1:1,000, 12,556 S; Cell Signaling Technology), collagen type I α1 (Col-I, 1:1,000, 72,026 T; Cell Signaling Technology), ALP antibody (1:1,000, AF2910; R&D Systems, USA), AKT (1:1,000, 60203-2-Ig; Proteintech, USA), phosphorylated (p)-AKT (1:1,000, 66444-1-Ig; Proteintech), PI3K (1:1,000, 20584-1-AP; Proteintech), PTEN (1:1,000, 22034-1-AP; Proteintech), CD9 (1:1,000, 60232-1-Ig; Proteintech), and CD63 (1:1,000, 67605-1-Ig; Proteintech), while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:5,000, 60004-1-Ig; Proteintech) was used as a loading control.

Techniques: Derivative Assay, Staining, Western Blot, Expressing, Comparison